抗ヒト唾液α-Amylase血清によるヒト唾液の免疫化学的証明に関する基礎的研究

概要

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Isolation and purification of α-amylase from human saliva were carried out by column chromatography on Sephadex G-25 and on DEAE-cellulose. A specific antiserum was obtained from rabbits by intramuscular administration of purified α-amylase mixed with equal volume of complete Freund's adjuvant. This antiserum showed positive reactions in agarose gel immunodiffusion against human saliva and purified α-amylase at the minimum protein concentration of 25 μg/ml and 10 μg/ml, respectively. The amylase prepared from human saliva, pancreas and Fallopian tube cyst showed different electrophoretic mobilities on thin-layer gel of agarose, but the antigenicity of each amylase preparation to the anti-α-amylase obtained above was identical. The anti-α-amylase inhibited 72 to 76% of the enzymatic activity of the amylases of human saliva, pancreas and Fallopian tube cyst fluid and 54 to 57% of the enzymatic activity of the amylases of human serum and urine. Human liver and seminal plasma amylases were not inhibited by this antiserum, suggesting that these enzymes are different structurally and immunologically from the amylases of human saliva, pancreas and Fallopian tube cyst fluid as well as human serum vand urine.
公益社団法人日本薬学会の論文
1969-10-31