ニトラゼパムの比色定量について

概要

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Micro-determination of nitrazepam was carried out. Nitrazepam solution was first made alkaline (pH 9-11) with 20% sodium carbonate solution and extracted with ethyl acetate by mechanical shaking. The ethyl acetate solution was then extracted with 10% hydrochloric acid, also by mechanical shaking, and the acid solution was heated at 100° for 15 minutes to effect hydrolysis. The product of hydrolysis, 2-amino-5-nitrobenzophenone, was colored by the Bratton-Marshall method and absorbance of the colored solution was measured at 535 mμ. This colorimetric determination was applicable to various samples containing nitrazepam, its recovery from the aqueous solution (91±5%) and urine (91±4%) was good, and showed good sensitivity, detection being possible in the range of 2-20 μg/ml concentration. Color of the azo pigment formed from N-(1-naphthyl) ethylenediamine dihydrochloride was stable, and it was considered that this method can be applied as a rapid and reliable determination of nitrazepam in forensic chemistry.
公益社団法人日本薬学会の論文
1970-12-31