ブロムワレリル尿素中毒の裁判化学的研究(第1報) : 薄層クロマトグラフィーによるブロムワレリル尿素および3-メチルブチリル尿素の検出

概要

論文の詳細を見る
The blood, internal organ and stomach-intestine content of bromvalerylurea ingested rabbit were extracted with acetone after lyophilization, whereas the urine was extracted with ether at pH 7.0 by Kutscher-Steudel Instrument. Each extract was concentrated under reduced pressure, and chromatographed on thin-layer plate using chloroform-acetone (9 : 1) and chloroform-methanol (9 : 1), and detected by KI-benzidine reagent. The Rf values and color reactions observed were as follows, bromvalerylurea (0.38,0.73,dark violet), 3-methylbutyrylurea (0.20,0.66,dark violet), and urea (0,0.03,orange). Bromvalerylurea and its metabolite in rabbit were analysed at intervals by the procedure described above. Both bromvalerylurea and 3-methylbutyrylurea were detected after 1-22 hours from stomachintestine content, internal organ (brain, little brain, spinal cord, liver, kidney, spleen, lung, bile), blood and after 48 hours, only 3-methylbutyrylurea from small intestine content, liver, kidney, spleen, lung, brain and urine. After 122 hours, no metabolite was found in almost all organs. Thus, it was recognized the bromvalerylurea was metabolized rapidly.
1969-08-30