Detection of Alcohol Dehydrogenase by Using NAD after Separation by Gel Electrophoresis
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概要
- 論文の詳細を見る
A facile detection method for alcohol dehydrogenase (ADH) in the gel after electrophoresis of tissue samples was developed. The gel plate was incubated in the presence of alcohol and nicotinamide adenine dinucleotide (NAD) and the resultant greenish fluorescent spots of NADH (reduced form of NAD) were detected under a 365-nm ultraviolet (UV) lamp. The gel plate was laid on a sheet of photographic paper and exposed to a flash of the UV light. From the photograph, the activity of ADH could be estimated. Aldehyde dehydrogenase (ALDH) was similary detectable with acetaldehyde as the substrate instead of alcohol.
- 公益社団法人日本薬学会の論文
- 1987-06-30
著者
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宮浦 修一
Faculty of Pharmaceutical Sciences, Teikyo University
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礒野 秀夫
Faculty of Pharmaceutical Sciences, Teikyo University
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礒野 秀夫
帝京大・薬
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宮浦 修一
Faculty Of Pharmaceutical Sciences Teikyo University
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礒野 秀夫
Faculty Of Pharmaceutical Sciences Teikyo University
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堀江 貴代子
Faculty of Pharmaceutical Sciences, Teikyo University
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堀江 貴代子
Faculty Of Pharmaceutical Sciences Teikyo University
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加藤 晴代
Faculty of Pharmaceutical Sciences, Teikyo University
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加藤 晴代
Faculty Of Pharmaceutical Sciences Teikyo University
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