Comparison of Hepatic Glutathione S-Transferases of Male and Female Rats

概要

論文の詳細を見る
Six and eight peaks of activity toward 1-chloro-2,4-dinitrobenzene (CDNB) were observed in carboxymethyl (CM)-cellulose chromatograms of hepatic glutathione S-transferases in female and male rats, respectively. These peaks were named transferases I, II, IIIa, III, IIIb, IV, V and VI in the order of elution with KCl (transferases IIIa and IIIb could not be detected in the case of female rats). Transferase I could be further resolved into at least two enzymes by CM-Sepharose CL-6B chromatography. Transferase II was confirmed to consist of YaYa subunits (ligandin) by CM-cellulose chromatography ; Habig et al. did not detect this transferase because of their use of 1,2-dichloro-4-nitrobenzene (DCNB) as a substrate for assaying these species of transferases. Phenobarbital treatment caused 43 and 93% increases in the total activity toward CDNB in female and male rat livers, respectively. Transferases I and II were strongly induced in both sexes of rats by this treatment. Transferases IV was slightly increased in both sexes. Transferases III and V were induced in male rats, but not in female rats. The subunit species and substrate specificities of the present transferases were compared with those of reported transferases.
社団法人日本薬学会の論文
1984-08-25