Influence of Phenobarbital on Growth of Chick Embryo Followed by Induction of Glutathione S-Transferase

概要

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The purpose of the present study was to learn the influence of phenobarbital (PB) on embryonic growth followed by induction of glutathione S-transferase activity (GST) by employing fertilized chicken eggs. A previous single dose (into the air-sac) of 168 μmol of PB killed all embryos tested by day 18. Those surviving when administered a dose of 144 μmol PB grew poorly and their hepatic color was changed to green. A curve of renal GST activity of 18-day-old embryo against PB dose linearly increased up to 120 μmol PB dose, where the activity was 6 times that in the control. A previous single dose (into the air-sac) of 60 μmol PB gave the following daily growth profiles : the whole body, the tibia and femoral bone grew poorly in weight and size after day 11 or 12. The hepatic weight did not differ from that of the control throughout the incubation period of day 7 to 18,however, the GST activity rose at day 14 to reach 2 times that in the control at day 18. The renal weight was slightly elevated compared with the control throughout the period of day 7 to 18,however, the GST activity linearly increased to reach 3 times that in the control at day 18. PB was found to induce hepatic GST activity of chick embryo causing such lesions as changing of the hepatic color to green.
公益社団法人日本薬学会の論文
1991-02-28