高速液体クロマトグラフィーによる生体試料中ブロムワレリル尿素の迅速定量法

概要

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A high-performance liquid chromatographic (HPLC) method for the quantitation of bromvalerylurea in biological materials is described. A cleanup of the samples for bromvalerylurea was achieved simply and rapidly by using Extrelut column with 1,2-dichloroethane. The detection limit was 5 ng on octadecylsilated silica column with a mobile phase of acetonitrile-10 mM phosphoric acid (22 : 78) and an ultraviolet detector (225 nm). The calibration curve gave a good linearity over the range of 0.5-50μg/ml and 20-1000μg/ml using phenobarbital as internal standard. The method also showed a good reproducibility, the coefficient of variation being 2.0% for blood, 6.8% for urine and 2.7% for brain. The contents of bromvalerylurea in various tissues of a suicide cadaver could be determined with this method. This simple and rapid analytical technique could be useful for the detection of bromvalerylurea from intoxicated subjects in the field of forensic toxicology and emergency medicine.
社団法人日本薬学会の論文
1986-10-31