生体中の微量金属の組織化学的染色法について

概要

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Recent studies on the histochemical staining of a metal in cells and tissues were reviewed mainly on the viewpoint of the mechanisms of metal staining by a chelating agent as follows, i) staining methods for a metal in cells and tissues, ii) molecule designing of a high sensitive chelating agent, iii) mechanisms of staining, iv) masking of interfering metals, v) metal staining in the future. With the modification of a chelating agent to increase the staining sensitivity, a molecular size, the selection of substituent groups and the introducing position of the groups to a chelator molecule, and the hydrolyzing property of metal chelates should be taken into consideration. The most frequently mentioned factors in a staining mechanism are dye-tissue attractive forces, and recent studies have make it clear that weak interactions, hydrogen bond and hydrophobic interaction, would play a significant role in those forces. The Hansch π values were an useful indication for knowing the hydrophobicity of chelating agents for metal staining. The published values of a stability constant were insufficient guide for choosing a masking agent to block interfering metals. The polyphosphates were among the most useful masking agents for metal staining from a practical standpoint. Future prospects about methods for histochemical metal staining developed recently are also referred.
公益社団法人日本薬学会の論文
1984-10-31