外用タール色素に関する衛生化学的研究(第1報)逆相分配高速液体クロマトグラフィーによる化粧品用アゾ色素の分析ならびに市販色素中の純色素の定量について

概要

論文の詳細を見る
Separation and determination of twenty kinds of cosmetic azo dyes permitted in Japan were studied by reversed-phase high performance liquid chromatography. For identification and determination of the tested dyes, the system of Nucleosil 5C_<18> column and acidic mobile phase was very useful. The used mobile phases were CH_3CN-THF-H_2O (pH 3.2,H_3PO_4) for oil-soluble dyes, and CH_3CN-THF-H_2O (pH2.0,HClO_4) for water-soluble dyes, Ca-lakes and Ba-lakes, respectively. This method was applied on 93 samples of commercial cosmetic azo dyes for the determination of "pure dye content." Data obtained by this method agreed well with those by the recommended official methods such as titanous chloride titration and spectrophotometric determination. Comparing with the official method, advantage of this method was to prevent the influences of contaminated other dyes, subsidiary dyes and impurities.
公益社団法人日本薬学会の論文
1983-10-31