Effect of Cetylmannoside Modification on the Alternative Complement Pathway Activation by Liposomes in Rat Serum
スポンサーリンク
概要
- 論文の詳細を見る
Complement activation is important for removing foreign substances by the mononuclear phagocyte system in vivo. The interaction between liposomes and complement components is considered to affect the clearance of liposomes from the circulation. It has been previously demonstrated in our laboratory that multilamellar vesicles (MLV) with surfaces modified by cetylmannoside (Man) were eliminated from the circulation rapidly and showed an approximately 2-fold higher hepatic accumulation compared with control MLV (PC-MLV) (Yamashita et al., Int. J. Pharmaceut., 70,225,1991). In this study, we investigated the effect of Man-modification on complement system activation. As far as elimination from the blood is concerned, the initial values of blood liposome concentration were decreased and liposomes were removed from the circulation rapidly in accordance with the extent of the Man content into their membranes. The Man-modification also affected the organ distribution of injected liposomes and their stability in rat serum. Except for MLV containing 50 mol% Man, it was observed that the hepatic uptake of liposomes was enhanced according to the increasing Man content, whereas splenic uptake was decreased and the splenic clearance was comparable. The stability of liposomes in rat serum decreased with increasing Man content. Liposomal instability in rat serum was significantly reduced by preheating the serum at 56℃ for 30 min, the treatment with anti-C3 antiserum and with EDTA but not abolished in serum treated with EGTA/MgCl_2. Thus, it is considered that the activation of the complement system through the alternative pathway is facilitated as a result of increasing the Man content in the liposomes. Furthermore, the contribution of the alternative complement pathway activation affected only the affinity of Man-modified MLV for the liver.
- 公益社団法人日本薬学会の論文
- 1995-04-15
著者
-
際田 弘志
Faculty Of Pharmaceutical Sciences University Of Tokushima
-
松尾 浩民
Faculty Of Pharmaceutical Sciences University Of Tokushima
-
山下 親正
Faculty Of Pharmaceutical Sciences Kyushu University
-
秋山 一恵
Faculty of Pharmaceutical Sciences, University of Tokushima
-
秋山 一恵
Faculty Of Pharmaceutical Sciences University Of Tokushima
-
際田 弘志
Faculty Of Pharmaceutical Sciences Tokushima University
-
Kiwada H
Faculty Of Pharmaceutical Sciences University Of Tokushima
関連論文
- Effects of Ascorbic Acid on Iproniazid-Induced Hepatitis in Phenobarbital-Treated Rats
- Studies on the Uptake Mechanism of Liposomes by Perfused Rat Liver. I. : An Investigation of Effluent Profiles with Perfusate Containing No Blood Component
- Effects of Fluidity and Vesicle Size on Antitumor Activity and Myelosuppressive Activity of Liposomes Loaded with Daunorubicin
- Application of Synthetic Alkyl Glycoside Vesicles as Drug Carriers. III. : Plasma Components Affecting Stability of the Vesicles
- Application of Synthetic Liposomes Based on Acyl Amino Acids or Acyl Peptides as Drug Carriers. I. Their Preparation and Transport of Glutathione into the Liver(Pharmaceutical)
- Studies on the Uptake Mechanism of Liposomes by Perfused Rat Liver. II. : An Indispensable Factor for Liver Uptake in Serum(Pharmaceutical)
- Feasibility of Magnetic Liposomes as a Targeting Device for Drugs
- Effects of Dose and Vesicle Size on the Pharmacokinetics of Liposomes
- Evaluation of Isonicotinoyl-γ-Aminobutyric Acid (GABA) and Nicotinoyl-GABA as Pro-drugs of GABA
- Effects of Glucose and Ascorbic Acid on Absorption and First Pass Metabolism of Isoniazid in Rats
- Uptake of Liposomes Surface-Modified with Glycyrrhizin by Primary Cultured Rat Hepatocytes
- Targeting of Liposomes Surface-Modified with Glycyrrhizin to the Liver. I. Preparation and Biological Disposition
- Effect of Cetylmannoside Modification on the Alternative Complement Pathway Activation by Liposomes in Rat Serum
- Tissue Distribution and Pharmacokinetic Evaluation of the Targeting Efficiency of Synthetic Alkyl Glycoside Vesicles
- Application of Synthetic Alkyl Glycoside Vesicles as Drug Carriers. I. Preparation and Physical Properties
- Correlations between in Vivo and in Vitro Dissolution Rates of (α-Bromoisovaleryl) urea Polymorphs
- Pharmacokinetic Study on the Polymorphs of (α-Bromoisovaleryl) urea in the Rat
- The Determination of (α-Bromoisovaleryl) urea in Plasma and the Bioavailabilities of Its Polymorphic Forms in the Rat
- The Dissolution Properties and Physico-chemical Properties of Polymorphic Forms of (α-Bromoisovaleryl) urea
- The Preparation and Properties of a New Polymorphic Form of (α-Bromoisovaleryl) urea
- The Pharmacokinetic Study on the Fate of 8-Hydroxyquinoline in Rat
- A New Numerical Calculation Method for Deconvolution in Linear Compartment Analysis of Pharmacokinetics
- Effect of Vesicle Size on in Vivo Release of Daunorubicin from Hydrogenated Egg Phosphatidylcholine-Based Liposomes into Blood Circulation
- ラットにおけるヒドラジンの脳内分布ならびにγ-アミノ酪酸上昇効果に関するより詳細なる研究(発表論文抄録(1983年))
- ラットにおけるヒドラジンの脳内分布ならびにGABA上昇効果(発表論文抄録(1983年))