Characterization of Lignoceroyl-CoA Ligase Activity in Chicken Liver Microsomes
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概要
- 論文の詳細を見る
Lignoceroyl-coenzyme A (CoA) ligase activity was detected in microsomal fractions from chicken liver in the presence of α-cyclodextrin as a solubilizing agent of lignoceric acid. Heptakis(2,6-di-O-methyl)-β-cyclodextrin (dimethyl-β-cyclodextrin) and hexakis(2,6-di-O-methyl)-α-cyclodextrin (dimethyl-α-cyclodextrin), among the cyclodextrins tested, were more effective than α-cyclodextrin as solubilizing agents. We have characterized lignoceroyl-CoA ligase activity in comparison with palmitoyl-CoA ligase activity. Lignoceroyl-CoA and palmitoyl-CoA ligase activities showed a similar dependency on CoA concentration. However, lignoceroyl-CoA ligase activity exhibited responses to the Mg^<2+> ion, adenosine triphosphate (ATP), ATP analogues (adenosine monophosphate (AMP) and adenosine diphosphate (ADP)), and heat treatment, which were distinctly different from the responses of palmitoyl-CoA ligase activity. These results are consistent with the idea that lignoceroyl-CoA and palmitoyl-CoA are synthesized by two different enzymes.
- 公益社団法人日本薬学会の論文
- 1993-10-15
著者
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宇田 裕
Department of Health Chemistry, Niigata College of Pharmacy
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白石 隆幸
Laboratory Of Health Chemistry Niigata College Of Pharmacy
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宇田 裕
Department Of Health Chemistry Niigata College Of Pharmacy
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宇田 裕
Laboratory Of Health Chemistry Niigata College Of Pharmacy
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