Purification and Characterization of α-L-Fucosidase from the Liver of Seahare, Aplysia kurodai

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概要

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• An α-L-fucosidase [EC 3.2.1.51] was isolated from liver of the marine gastropod, Aplysia kurodai. The enzyme was purified by procedures involving extraction, ammonium sulfate precipitation, and chromatographies on diethylaminoethyl (DEAE)-Sephadex, hydroxylapatite and Sepharose 6B. The final preparation was sufficiently free from other glycosidase activities and gave a single protein band on disc gel electrophoresis. The molecular weight of the enzyme was estimated to be 330000 by Sephadex G-200 column chromatography. The enzyme has two optimal pH values, one at pH 3.0 and the other at pH 6.4,but it was stable only in the pH range from 4.5 to 6.0. The enzyme released fucose not only from p-nitrophenyl α-L-fucoside but also from human milk oligosaccharides.

• 公益社団法人日本薬学会の論文
• 1985-04-25

著者

• 宇田 裕

  Department of Health Chemistry, Niigata College of Pharmacy

• 宇田 裕

  Department Of Health Chemistry Niigata College Of Pharmacy

• 宇田 裕

  Laboratory Of Health Chemistry Niigata College Of Pharmacy

• 平岩 雅男

  Laboratory Of Health Chemistry Niigata College Of Pharmacy

関連論文

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• Substrate Specificities of α-N-Acetylgalactosaminidases I and II from Squid Liver
• Enzymatic Analysis of Cyanogenic Glycosides. II. : A Simple Method by Using a Microdiffusional Apparatus
• Characterization of Lignoceroyl-CoA Ligase Activity in Chicken Liver Microsomes
• Purification and Characterization of α-L-Fucosidase from the Liver of Seahare, Aplysia kurodai

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