Properties and Human Origin of Two Angiotensin-I-Converting Enzyme Inhibitory Peptides Isolated from a Tryptic Hydrolysate of Human Serum Albumin

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概要

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• Two angiotensin-I-converting enzyme (ACE) inhibitory peptides were isolated from a tryptic hydrolysate of human serum albumin (HSA). The peptides were identified by sequencing and other analyses as Ala-Trp and the nonapeptide Ala-Phe-Lys-Ala-Trp-Ala-Val-Ala-Arg (human albutensin A), corresponding to f(213-214) and f(210-218) of HSA, respectively. Synthetic versions of both peptides had previously been shown to have ACE inhibitory activity. The present results are the first to show that these peptides have a potential natural origin in humans. Additional studies were done to define the inhibitory properties of these peptides, as they had not been previously reported. The dipeptide and nonapeptide showed dose-dependent inhibition of ACE, with IC_<50> values of 12 and 1.7 μmol/l, respectively. Lineweaver-Burk plots suggested that Ala-Trp is a competitive inhibitor, and that human albutensin A is a noncompetitive inhibitor.

• 公益社団法人日本薬学会の論文
• 2000-07-01

著者

• AKIZAWA Toshifumi

  Faculty of Pharmaceutical Sciences, Setsunan University

• TANIMURA Takenori

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• Sadakane Yutaka

  Faculty Of Pharmaceutical Sciences Kyushu University Of Health And Welfare

• Nakagomi K

  Department Of Analytical Chemistry School Of Pharmaceutical Sciences Teikyo University

• Nakagomi Kazuya

  Faculty Of Pharmaceutical Sciences Teikyo University

• Tanimura Takenori

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

• EBISU Hidetoshi

  Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

• FUJII Noriko

  PRESTO, JST and Research Reactor Institute, Kyoto University

• Ebisu Hidetoshi

  Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University

• Sadakane Y

  Department Of Analytical Chemistry School Of Pharmaceutical Sciences Kyushu University Of Health And

• Akizawa Toshifumi

  Faculty Of Pharmaceutical Sciences Setsunan University

• Nakagomi Kazuya

  Department Of Analytical Chemistry School Of Pharmaceutical Sciences Teikyo University

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