Labeling Conditions Using a 2-Aminobenzamide Reagent for Quantitative Analysis of Sialo-oligosaccharides
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概要
- 論文の詳細を見る
During 2-aminobenzamide (2-AB)-labeling of sialo-oligosaccharides the authors encountered a significant loss of sialic acids under the reported conditions, at 65℃ for 2 h. By examination of the relationship between labeling temperature and recovery of tetra-sialylated oligosaccharides, 2-AB-labeling at 37℃ for 16 h resulted in a maximum yield with practically no loss of sialic asid.Under the newly optimized conditions, the reproducibility of 2-AB-labeling was examined in triplicate with three lots of recombinant human erythropoietin (r-HuEPO). The present conditions gave analytical date comparable to those obtained by the established NaB[^3H]_4 labeling procedure. The modified method has been applied to oligosaccharide structural analysis of sialoglycoprotein therapeutics.
- 公益社団法人日本薬学会の論文
- 2000-03-01
著者
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Takeuchi Makoto
Chemistry Research Laboratories Astellas Pharmaceutical Inc.
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Inoue Noboru
Chemistry And Quality Control Group Pharmaceutical Development Laboratory Kirin Brewery Co. Ltd.
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WATANABE Toshinori
Chemistry and Quality Control Group, Pharmaceutical Development Laboratory, Kirin Brewery Co., Ltd.
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KUTSUKAKE Toshiko
Glycotechnology Group, Central Laboratory Kirin Brewery Co., Ltd.
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MATSUKI Shigeru
Chemistry and Quality Control Group, Pharmaceutical Development Laboratory, Kirin Brewery Co., Ltd.
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TAKEUCHI Makoto
Glycotechnology Group, Central Laboratory Kirin Brewery Co., Ltd.
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Matsuki Shigeru
Chemistry And Quality Control Group Pharmaceutical Development Laboratory Kirin Brewery Co. Ltd.
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Kutsukake Toshiko
Glycotechnology Group Central Laboratory Kirin Brewery Co. Ltd.
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Takeuchi Makoto
Glycotechnology Group Central Laboratory Kirin Brewery Co. Ltd.
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Watanabe Toshinori
Chemistry And Quality Control Group Pharmaceutical Development Laboratory Kirin Brewery Co. Ltd.
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