Simultaneous Determination of the Lactone and Carboxylate Forms of 7-Ethyl-10-hydroxycamptothecin (SN-38), the Active Metabolite of Irinotecan (CPT-11), in Rat Plasma by High Performance Liquid Chromatography
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概要
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We established a high performance liquid chromatography (HPLC) method for the simultaneous determination of the lactone and carboxylate forms of 7-ethyl-10-hydroxycamptothecin (SN-38), the active metabolite of the antitumor drug irinotecan (CPT-11), in rat plasma. Plsma samples were pretreated with chilled MeOH and zinc sulfate to precipitate protein, and were then directly injected into the HPLC system. Chromatography was carried out with a Puresil C18 column (particle size 5 μm), and the mobile phase consisted of 0.1 M ammonium acetate buffer (pH 5.5) and acetonitrile (70/30,v/v) containing 20 mM of tetra-n-pentylammonium bromide. The column effluent was monitored with a spectrofluorometer (excitation wavelength 380 nm, emission wavelength 540 nm). The method was valid for SN-38 lactone (5-2500 ng/ml) and carboxylate (5-1000 ng/ml).
- 公益社団法人日本薬学会の論文
- 1997-07-15
著者
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Kaneda Norimasa
Yakult Central Institute For Microbiological Research
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Yokokura T
Yakult Central Inst. Microbiological Res. Tokyo Jpn
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Yokokura Teruo
Yakult Central Institute For Microbiological Research
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Yokokura Teruo
Yakult Institute For Microbiological Research
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Yokokura Teruo
Yakult Cent. Inst. Miorobiol.
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Kaneda N
Pharmacognosy Kobe Pharmaceutical University
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Hosokawa Yugo
Yakult Central Institute For Microbiological Research
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HOSOKAWA Yukiko
Yakult Central Institute for Microbiological Research
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