Effects of Gentamicin Sulfate on Enzyme Activities of Carbonic Anhydrase from Human Erythrocytes in Vitro and from Rat Erythrocytes in Vivo

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概要

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• The effects of gentamicin sulfate on carbonic anhydrase (CA) enzyme activity in in vitro human and in in vivo rat erythrocytes were investigated. For in vitro study, human carbonic anhydrase-I and -II (HCA-I and HCA-II) were purified by affinity-column chromatography, and rats were used for in vivo study. In vivo and in vitro CA enzyme activity was determined colorimetrically using the CO_2-hydration method of Wilbur and Anderson as modified by Rickli et al Gentamicin sulfate (1.98-9.90 mM) showed in vitro inhibitory effects on HCAI and HCA-II hydratase activity up to a 2 mM concentration, when determined using the CO_2-hydratase method. Rat erythrocyte CA activity was significantly inhibited for up to 3 h (p<0.001) following intramuscular administration of gentamicin sulfate to Sprague-Dawley rats (3.2 mg/kg body weight). In conclusion, gentamicin sulfate inhibits CA enzyme activity in vivo and at low concentrations in vitro, but activated it at high concentrations (≥4 mM) in vitro.

• 公益社団法人日本薬学会の論文
• 2002-08-01

著者

• Beydemir Sukru

  Ataturk University Faculty Of Science And Arts Department Of Chemistry

• BUYUKOKUROGLU Mehmet

  Ataturk University, Medical Faculty, Department of Pharmacology

• CIFTCI Mehmet

  Ataturk University, Biotechnology Application and Research Center

• KUFREVIOGLU Omer

  Ataturk University, Faculty of Science and Arts, Department of Chemistry

• Buyukokuroglu Mehmet

  Ataturk University Medical Faculty Department Of Pharmacology

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• Effects of Gentamicin Sulfate on Enzyme Activities of Carbonic Anhydrase from Human Erythrocytes in Vitro and from Rat Erythrocytes in Vivo
• Morphine Inhibits Erythrocyte Carbonic Anhydrase in Vitro and in Vivo(Biochemistry)
• In Vitro and in Vivo Effects of Dantrolene on Carbonic Anhydrase Enzyme Activities (Biochemistry/Molecular Biology)

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