Morphine Inhibits Erythrocyte Carbonic Anhydrase in Vitro and in Vivo(Biochemistry)

概要

論文の詳細を見る
Morphine is implicated in diverse functions, from development to immune modulation in the central and peripheral nervous systems. At the present time, morphine is one of the most effective antinociceptive agents used to manage pain. It has been used extensively in the clinical management of pain due to its potent analgesic effect. In this study, the in vitro and in vivo inhibitory effects of morphine on erythrocyte carbonic anhydrase (CA) were investigated. Human erythrocyte isoenzymes, HCA-I and HCA-II, were purified by Sepharose-4B affinity chromatography column with a yield of 66.95 and 62.82%, a specific activity of 3892.3 and 11663.2 EU/mg proteins with 745.1 and 2232.6-fold purification of each isoenzyme, respectively. To determine enzyme purity, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed. In vitro inhibition of erythrocyte HCA-I and HCA-II by morphine using the CO_2-hydratase enzyme gave IC_<50> values 4.50×10^<-5>M (γ^2:0.954) and 9.23×10^<-5>M (γ^2:0.996), respectively. CA activity was significantly attenuated in vivo in Spraque-Dawley rats for up to 3h (p<0.001) following intraperitoneal administration of morphine. In conclusion, morphine inhibited CA activity both in vitro and in vivo.
公益社団法人日本薬学会の論文
2007-12-01