In Vitro and in Vivo Effects of Dantrolene on Carbonic Anhydrase Enzyme Activities (Biochemistry/Molecular Biology)

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概要

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• The effects of dantrolene were investigated on carbonic anhydrase (CA) enzyme activities in in vitro human and in in vivo Sprague-Dawley rat erythrocytes. For in vitro study, human carbonic anhydrase-I (HCA-I) and -II (HCA-II) were purified by Sepharose 4B-L-tyrosine-sulfanylamide affinity chromatography, rats were used for in vivo study. In vivo and in vitro CA enzyme activity was determined colorimetrically using the CO_2-hydration method of Wilbur and Anderson. Dantrolene (1.64×10^<-5>-6.56×10^<-5>M) showed in vitro inhibitory effects on HCA-I and HCA-II hydratase activity, when determined using the CO_2-hydratase method. 50% inhibitory concentration (IC_<50>) was 4.09×10^<-5>M for HCA-I and 3.24×10^<-5>M for HCA-II. Rat erythrocyte CA activity was significantly inhibited by 10 mg/kg dantrolene for up to 3 h (p<0.001) following intraperitoneal administration. In conclusion, Dantrolene inhibited the carbonic anhydrase enzyme activity under in vitro and in vivo conditions.

• 公益社団法人日本薬学会の論文
• 2004-05-01

著者

• Beydemir S

  Ataturk University Science And Arts Faculty Department Of Chemistry

• Beydemir Sukru

  Ataturk University Faculty Of Science And Arts Department Of Chemistry

• BUYUKOKUROGLU Mehmet

  Ataturk University, Medical Faculty, Department of Pharmacology

• GULCIN Ilhami

  Ataturk University, Science and Arts Faculty, Department of Chemistry

• Buyukokuroglu Mehmet

  Ataturk University Medical Faculty Department Of Pharmacology

• Buyukokuroglu Mehmet

  Ataturk University Medicine Faculty Department Of Pharmacology

• Gulcin Ilhami

  Ataturk University Science And Arts Faculty Department Of Chemistry

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• In Vitro and in Vivo Effects of Dantrolene on Carbonic Anhydrase Enzyme Activities (Biochemistry/Molecular Biology)

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