Isoelectric Points of Two Molecular Forms of Human Urinary Urokinase determined by Density-gradient Isoelectric Focusing and Isotachoelectrophoresis

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概要

• 論文の詳細を見る

• The isoelectric points (pI's) of MW 55000 form (H-UK) and MW 36000 form (L-UK) of human urinary urokinase (UK) were determined by sucrose density-gradient isoelectric focusing. These values were compared with the pI's which had been estimated previously by isotachoelectrophoresis. All five subforms fractionated from H- or L-UK by isoelectric focusing were confirmed to exhibit plasminogen activator activity and to be homogeneous in terms of molecular weight. The pI's evaluated by isoelectric focusing were in accordance with those by isotachoelectrophoresis, for all five subforms of H-UK (pI 8.70,8.86,9.05,9.20 and 9.44) and for three subforms of L-UK (pI 7.54,8.26 and 8.84). The pI's of the two most alkaline subforms of L-UK (pI 9.17 and 9.43) were underestimated by up to 0.3 pH unit by isoelectric focusing as compared with the values obtained by isotachoelectrophoresis (pI 9.4 and 9.7,respectively). Thus, the differences between the pI's determined by the two kinds of electrophoresis were slight and restricted to two of the ten subforms.

• 公益社団法人日本薬学会の論文
• 1982-04-25

著者

• 鈴木 章

  Traditional Chinese Medicine Research Laboratory Kanebo Co. Ltd.

• 三羽 信比古

  Pharmaceuticals Research Center Kanebo Ltd.

• 沢田 照夫

  Pharmaceuticals Research Center Kanebo Ltd.

• 鈴木 章

  Pharmaceuticals Research Center, Kanebo, Ltd.

関連論文

• Determination of Particle Size Distribution of Fluorocarbon Emulsion by Means of Centrifugal Sedimentation : A Proposal for Specifying the Particle Size Distribution
• Isoelectric Points of Two Molecular Forms of Human Urinary Urokinase determined by Density-gradient Isoelectric Focusing and Isotachoelectrophoresis
• Alteration of Polypeptide Backbone Structure as determined by Circular Dichroic Spectral Analysis in Relation to Change of Plasminogen Activator Activity of Two Forms of Human Urinary Urokinase denatured with Guanidine Hydrochloride
• Comparative Studies on Two Active Enzyme Forms of Human Urinary Urokinase. II. pH- and Heat-stabilities of Plasminogen Activator Activity
• Comparative Studies on Two Active Enzyme Forms of Human Urinary Urokinase. I. Purification by Serial Column Chromatography and Homogeneity Analyses of Molecular Weight and Isoelectric Point
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