Existence of Three Forms of H_2M_2 Isoenzyme of Lactate Dehydrogenase

概要

論文の詳細を見る
Lactate dehydrogenase (LDH) was purified from rat and bovine tissues by affinity chromatography on immobilized colchicine and used for the separation of isoenzymes by high-performance anion-exchange liquid chromatography (HPLC). This analysis showed the splitting of rat H_2M_2 into three peaks and of bovine H_2M_2 into two peaks. The heat stability, inactivation rate of urea and electrophoretic mobility of isoenzymes were examined and these analyses indicated differences in physicochemical properities for the respective peaks of rat and bovine H_2M_2. In hybridization experiments, the splitting of H_2M_2 into three peaks was achieved only with the combination of rat H_4 and rat M_4,while the other combinations of bovine H_4 and bovine M_4,of rat H_4 and M_4 and of bovine H_4 and rat M_4 resulted in two H_2M_2 peaks.These results demonstrate that H_2M_2 of LDH in normal rat and bovine tissues is always split into two or three peaks by HPLC and that these H_2M_2 peaks have different physicochemical propertites, suggesting the existence of three possible geometrical isomers of H_2M_2.
公益社団法人日本薬学会の論文
1990-12-25