ろ紙電気泳動法による法定食用水溶性タール色素の同定法と粉末ジュースの試験成績について

概要

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A simple and rapid method to test water soluble coal-tar food colours legally permitted in Japan was studied using alumina column chromatography and paper electrophoresis and the following results were obtained. (1) As the buffer solution for the paper electrophoresis 5N acetic acid was most advantageous, because of inconsiderable tailing and minimized variation of relative mobilities to flavianic acid. (2) In order to identify the dyes which were difficult to identify under above mentioned condition or to increase the certainty of the identification of the other dyes, 1% borax solution was used in place of 5N acetic acid as buffer solution to give good results. (3) Mobilities of dyes in paper electrophoresis were effected with ambient temperature, but the relative mobilities to flavianic acid did not vary. (4) The application of this method to the dyes contained in powdered juices on the market gave satisfactory results, and this method could be applied to test the sample in which five colours were mixed. (5) Isolation of the dyes from the powdered juice was carried out by the alumina column chromatographic method under the mild condition, so this will be applicable to the unstable substances.
公益社団法人日本薬学会の論文