Properties of a New Alkaline Proteinase from Aspergillus niger

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概要

• 論文の詳細を見る

• A. niger LCF 9 synthesizes a new aspergillopeptidase of potential interest in therapeutics. The properties and operating range of the enzyme were determined. It is a semi-alkaline aspergillopeptidase (EC 3.4.23.4) with one endopeptidase activity. Its pI is 4.10,its molecular weight is 21000 Da and its A^<1%>_<1cm> at 280 nm is 9.75. It rapidly hydrolyzes casein and hemoglobin. Its optimal pH is 7.8 and optimal temperature is 45℃. It is thermally labile above 40℃ but can be stabilized by adding calcium ions. It is inhibited by phenylmethylsulfonyl fluoride (PMSF), by ethylenediaminetetraacetic acid (EDTA) and by certain metals ions, e.g. copper, manganese and cobalt ions. It has no dipeptidase or tripeptidase activity and its esterase activity is weak. It has a high collagenase activity and is to our knowledge the only aspergillopeptidase that is active toward benzoyl-arginine p-nitroanilide (BAPNA).

• 公益社団法人日本薬学会の論文
• 1989-05-25

著者

• POURRAT Aimee

  Laboratoire de Pharmacie Galenique et Pharmacotechnie Industrielle

• POURRAT HENRI

  Laboratoire de Pharmacie Galenique et Pharmacotechnie

• Pourrat A

  Laboratoire De Pharmacie Galenique Et Pharmacotechnie Industrielle

• Pourrat H

  Laboratoire De Pharmacognosie Et Biotechnologie

• BARTHOMEUF Chantal

  Laboratoire de Pharmacognosie et Biotechnologie

• Barthomeuf C

  Laboratoire De Pharmacognosie Et Biotechnologie

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• Stabilization of Octastation, a Somatostatin Analogue. Preparation of Freeze-Dried Products for Parenteral Injection
• Collagenolytic Activity of a New Semi-Alkaline Protease from Aspergillus niger
• Properties of a New Alkaline Proteinase from Aspergillus niger
• Production of Semi-Alkaline Protease by Aspergillus niger
• Production of Galilc Acid from Tara by a Strain of Aspergillus niger
• Production, Purification and Characterization of a Tannase from Aspergillus niger LCF 8
• Production of Inulinase by a New Mold of Penicillium rugulosum

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