Production, Purification and Characterization of a Tannase from Aspergillus niger LCF 8

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概要

• 論文の詳細を見る

• Tannase (Tannin acyl hydrolase EC 3.1.1.20) is produced in high yield by Aspergillus niger LCF 8. Optimal conditions of production were determined. Induction of teh strain, pH and aeration are the main parameters in fluencing the production yield. A pure enzyme sample (1,980 nKat/mg protein) was obtained by physical disruption of the mycellium, ultrafiltration and high pressure size exclusion chromatography (HPSEC). The biochemical properties of the enzyme were investigated. It is a glycoprotein of about 186,000 Dal, containing 43% sugars. The pI is 4.3. The optimal temperature for activity was 35℃. Esterase activity peaked at pH 5.0 while tannase activity was optimal at pH 6.0. The tannase was stable between pH 3.5 and 8.0 at temperature below 50℃. An in situ immobilized enzyme was also obtained. This was highly active towards gallotannins and ellagitannins. Since A. niger is officially approved in numerous countried for enzyme production in the food industry, the in situ immobilised tannase can be used for removing tannins responsible for unwanted effects in food or cosmetology processing.

• 公益社団法人日本生物工学会の論文
• 1994-03-25

著者

• POURRAT HENRI

  Laboratoire de Pharmacie Galenique et Pharmacotechnie

• Pourrat Henri

  Laboratoire De Pharmacohnosie Et De Biotechnologie Faculte De Pharmacie

• BARCHOMEUF CHANTAL

  Laboratoire de Pharmacognosie et de Biotechnologie, Faculte de Pharmacie

• REFERAT FANCOISE

  Labotatorie de Pharmacognosie et de Biotechnologie, Faculte de Pharmacie

• Barchomeuf Chantal

  Laboratoire De Pharmacognosie Et De Biotechnologie Faculte De Pharmacie

• Referat Fancoise

  Labotatorie De Pharmacognosie Et De Biotechnologie Faculte De Pharmacie

関連論文

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• Stabilization of Octastation, a Somatostatin Analogue. Preparation of Freeze-Dried Products for Parenteral Injection
• Collagenolytic Activity of a New Semi-Alkaline Protease from Aspergillus niger
• Properties of a New Alkaline Proteinase from Aspergillus niger
• Production of Semi-Alkaline Protease by Aspergillus niger
• Production, Purification and Characterization of a Tannase from Aspergillus niger LCF 8
• Production of Inulinase by a New Mold of Penicillium rugulosum

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