Determination of Sisomicin in Eluate from Dried Blood Spot on Filter Paper Disc for Monitoring of Blood Level in Rat, be Reversed-Phase High-Performance Liquid Chromatography after Pre-column Fluorimetric Derivatization
スポンサーリンク
概要
- 論文の詳細を見る
About 20 μl of whole blood obtained by venipuncture from rat tail vein was spotted onto a filter paper and the blood spot was punched out (5 mm diameter). Sisomicin (SISO) in the dried blood spot (DBS) was extracted effectively into 0.5M Na_2HPO_4 solution by ultrasonication and determined by reversed-phase high-performance liquid chromatography with pre-column derivatization using o-phthalaldehyde and β-mercaptopropionic acid. This method could be used for the pre-clinical study of SISO blood levels of a number of mice or rats without killing. The results were identical with those for SISO in serum, if corrected for hematocrit values, and were used for the calculation of pharmacokinetic parameters for individual rats. The detection limit of SISO in DBS (10.1 μl of whole blood) was 1.0 μg per ml of whole blood.
- 公益社団法人日本薬学会の論文
- 1989-01-25
著者
-
藤本 尚
Research & Development Lab. Essex Nippon Kabushiki Kaisha
-
広瀬 信吾
Kyoto Pharmaceutical University
-
田和 理市
Kyoto Pharmaceutical University
関連論文
- Stability of Urokinase in Solutions Containing Sodium Bisulfite
- Stability Prediction of Nafamostat Mesilate in an Intravenous Admixture Containing Sodium Bisulfite
- Degradation of Gabexate Mesilate by Sodium Bisulfite
- THE FORMATION OF 3-HYDROXY-4-METHOXYPHENYLALANINE AND 3-HYDROXY-4-METHOXYPHENETHYLAMINE IN PLASMA DURING L-DOPA THERAPY IN PATIENTS WITH PARKINSON'S DISEASE
- THE FORMATION OF 3-HYDROXY-4-METHOXYPHENYLALANINE AND 3-HYDROXY-4-METHOXYPHENETHYLAMINE IN PLASMA DURING L-DOPA THERAPY IN PATIENTS WITH PARKINSON'S DISEASE
- Determination of Some Basic Samples in Anhydrous Acetic Acid by Complementary Tristimulus Colorimetry
- Continuous Fluorescence Detection of Creatine Phosphokinase Isoenzymes by the Use of a Stream-Switching Valve
- High Performance Liquid Chromatographic Separation and Detection of Methoxy Derivatives of 3,4-Dihydroxyphenylalanine
- Formation of 3-Hydroxy-4-methoxyphenylalanine from 3,4-Dihydroxyphenylalanine by Rat Liver Homogenate
- Microscopic Acid Dissociation Constants of 3,4-Dihydroxyphenylbutyric Acid and 3,4-Dihydroxybenzoic Acid
- Microscopic Acid Dissociation Constants of 3,4-Dihydroxyphenethylamine (Dopamine)
- Determination of Sisomicin in Eluate from Dried Blood Spot on Filter Paper Disc for Monitoring of Blood Level in Rat, be Reversed-Phase High-Performance Liquid Chromatography after Pre-column Fluorimetric Derivatization
- Fluorometric Determination of Sisomicin, an Aminoglycoside Antibiotic, in Dried Blood Spots on Filter Paper by Reversed-Phase High-Performance Liquid Chromatography with Pre-column Derivatization
- An On-line Clean-up Procedure for Large Sample Volume Analysis of Serum Aminoglycoside Antibiotics by Reversed-Phase High-Performance Liquid Chromatography
- Acid Dissociation of Tyrosine and Its Related Compounds
- Relationship between the Color Transition and the Equivalence Point of Methylrosaniline Chloride in Non-aqueous Titration
- Simultaneous Assay of hypoxanthine, Xanthine and Allopurinol by High-Performance Liquid Chromatography and Activation of Immobilized Xanthine Oxidase as an Enzyme Reactor
- Analysis of Binary Mixtures of Tertiary Amines by Measurement of the Decomposition Rates of π-Complexes with Tetracyanoethylene
- Determination of Kinetic Parameters for Immobilized Xanthine Oxidase and Its Application to a Post-Column Enzyme Reactor in High-Performance Liquid Chromatographic Analysis of Hypoxanthine, Xanthine and Uric Acid
- A Statistical Method for the Determination of Serum Uric Acid
- Differential Kinetic Determination of Mixtures of Aniline and Its Derivatives Using N, N-Dimethyl-p-phenylenediamine
- Determination of Some Tertiary Amines as π-Complexes with Tetracyanoethylene
- Indirect Micro-determination of Primary and Secondary Amines by Copper (II)-catalyzed Oxidation of Pyrocatechol Violet