Fluorometric Determination of Sisomicin, an Aminoglycoside Antibiotic, in Dried Blood Spots on Filter Paper by Reversed-Phase High-Performance Liquid Chromatography with Pre-column Derivatization
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概要
- 論文の詳細を見る
A simple method for the determination of sisomicin (SISO) in dried blood spots (DBS) on filter paper has developed. SISO in DBS was recovered most effectively by ultrasonication in 0.05M KH_2PO_4-borate buffer (pH 9.0). The eluates from the DBS were treated with a carboxymethyl-Sephadex column, followed by determination be reversed-phase high-performance liquid chromatography after pre-column fluorescence derivatization using o-phthalaldehyde and β-mercaptopropionic acid in 0.05M KH_2PO_4-borate buffer (pH 9.0). The detection limit of SISO was below 0.05μg per ml of whole blood (100μl). The method permits a simple blood collection method for monitoring of aminoglycoside antibiotics at therapeutic levels.
- 公益社団法人日本薬学会の論文
- 1988-04-25
著者
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藤本 尚
Research & Development Lab. Essex Nippon Kabushiki Kaisha
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広瀬 信吾
Kyoto Pharmaceutical University
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田和 理市
Kyoto Pharmaceutical University
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