Chemical Characteristics of D-I, a Hypotensive Factor Occurred in Acetone Extract of Bovine Brain

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A modified procedure for preparation of D-I was offered, and became possible preparation of highly purified material showing a single spot on thin-layer chromatography in a more effective manner. Further examinations on chemical characteristics of D-I involving assessments of functional groups by chemical inactivations treating with various agents were carried out. In Girard's separation, about 30% of depressor-activity was recovered in nonketonic fraction. By treatment with sodium borohydride, the hypotensive activity disappeared completely. It was abolished completely with treatment by hydrogen peroxide (0.1%) or permanganate, while the activity was recovered almost completely by treatment with ferric chloride. It was also damaged with bromine. D-I became substantially less active by treatment with succinic anhydride, and inactive by phenylisocyanate. By treatment with triphenylphosphine or diazomethane at cold D-I was unaffected. The attitudes of D-I against several spray reagents on TLC were further examined, and it was found that 2,4-dinitrophenylhydrazine-ferricyanide, 2', 7'-dichlorofluorescein and others were useful for detection of D-I. Generally, absorptional pattern of D-I in infrared spectrum resembled that of lysophosphatidyl choline. D-I might be a choline-containing phospholipid having double bond, free hydroxyl, ester, whereas it has no free amino, free carboxyl, amide, peroxide nor vicinal hydroxyl group, and it might have no carbonyl group other than ester. However, it might be convertible readily to a ketonic compound by some causes.
公益社団法人日本薬学会の論文
1978-11-25

Chemical Characteristics of D-I, a Hypotensive Factor Occurred in Acetone Extract of Bovine Brain

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