Comparison of Biological and Immunological Methods for Determination of Serum α_1-Antitrypsin

概要

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Comparison of biological and immunological methods for determination of α_1-antitrypsin showed that the amount of α_1-antitrypsin determined in the presence of trypsin were not the same. From the result of the comparison of effect of heat on the amount of α_1-antitrypsin found by biological and immunological methods, it is suggested that α_1-antitrypsin and the α_1-antitrypsin-trypsin complex are indistinguishable immunologically. The investigation of the trypsin inhibitory activity of α_1-antitrypsin and molecular weight determination showed that α_1-antitrypsin and trypsin formed a 1 : 1 stoichiometric complex. The results of immunoelectrophoresis and sodium dodecyl sulfate electrophoresis indicated that the α_1-antitrypsin-trypsin complex was broken down with incubation and the breakdown products also had immunogenicity.
社団法人日本薬学会の論文
1978-01-25