Medical and Biochemical Application of Infrared Spectroscopy. VI. Studies on Human Bile by Infrared Spectra and Some of its Clinical Application.

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Infrared spectral measurement was introduced into the analysis of bile and this was proved to be the new, simple, and effective tool for clinical tests. 1) Interpretation of the infrared spectrum of dried whole bile was established and a method was revealed for finding quantitative difference of various components of bile from its absorptions. Some examples of clinical application for the detection of abnormal bile was described, 2) Bile acids, bile pigments, bile lipids, and mucoic substances were separated from bile by a simple procedure and the method was revealed to find quantitative changes in the components constituting each of these fractions from their infrared spectra. 3) This infrared spectral analysis was found to be a new and simple method for finding the kind of bile acids present, which had been difficult by the known methods. It was found that the main component of bile acids in normal human bile is cholic acid and not deoxycholic acid.
1962-12-25