クロマトグラフィーの応用(第24報)サントニンとその同族体のクロマトグラフィーによる分離証明

概要

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Synthetic santonin, its stereoisomers and carboxylic acid ester intermediates, which are all neutral compounds, were developed using dioxane-water (4 : 6) and petroleum ether-dioxane (70 : 6) as the stationary and mobile phases, respectively, and the resulting bands were detected by ultraviolet light (254mμ) as light absorption spots or by printing the spots on a sensitized paper. In this case, the volume ratio between stationary phase and mobile phase on paper must remain within a definite range. The change of the ratio at various room temperatures and relative humidities were investigated in detail, using Toyo filter paper No.131. The acid compounds obtained in the course of the synthesis of santonin and its isomers were well separated when developed with alcoholic solvent of various percentages or with butanol satura ed with 3% ammonia water. Of the acid compounds, dl-11-carboxy-3-oxoeusantona-1,4-dienic acid gave two bands of its optically active components when developed on a paper impregnated with brucine. Paper chromatography of the brucine salt of this compound also gave the same result. Of the above two bands, the one with smaller Rf value was in complete agreement with that obtained by developing the brucine salt of d-11-carboxy-3-oxoeusantona-1,4-dienic acid.
公益社団法人日本薬学会の論文
1955-06-20