Relationship between Effects of Phenolic Compounds on the Generation of Free Radicals from Lactoperoxidase-Catalyzed Oxidation of NAD(P)H or GSH and their DPPH Scavenging Ability

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概要

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• The influence of various phenolic compounds on the lactoperoxidase(LPO)/hydrogen peroxide(H_2O_2), catalyzed oxidation of biochemical reductants such as reduced β-nicotinamide adenine dinucleotide(NADH), reduced β-nicotinamide adenine dinucleotide phosphate(NADPH)or reduced glutathione(GSH)was investigated by electron spin resonance(ESR)spectroscopy. Micromolar quantities of phenolic compounds such as 17β-estradiol, phenol, and p-chlorophenol enhanced the LPO/H_2O_2-catalyzed oxidation of NAD(P)H or GSH to generate a large amount of superoxide radical(O^<・->_2)or glutathione thiyl radical(GS^・), while, phenolic compounds such as quercetin and Trolox C greatly suppressed the generation of O^<・->_2 and GS^・, their quenching activities for a stable radical, 1, 1-diphenyl-2-picrylhydrazyl(DPPH), were investigated by ESR spectroscopy. 17β-Estradiol, phenol, and p-chlorophenol showed very weak scavenging activities for DPPH, but querectin and Trolox C showed strong activities. This suggests that the ability of phenolic compounds to enhance LPO/H_2O_2-catalyzed oxidation of NA(P) or GSH relates inversely to their ability to quench DPPH. That is, phenolic compounds having weak quenching activity aganist DPPH may enhance the LPO/H_2O_2-catalyzed oxidation of NAD(P)H or GSH to generate a large amount of O^<・->_2 or GS^・.

• 社団法人日本薬学会の論文
• 2001-03-01

著者

• Ueda Jun-ichi

  National Institute Of Radiological Sciences

• TSUCHIYA Yuki

  National Institute of Radiological Sciences

• OZAWA Toshihiko

  National Institute of Radiological Sciences

• Ozawa Toshihiko

  National Institute Of Radiological Science

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