Kinetic Comparisons of Anthocyanin Reactivities towards 2, 2'-Azobis (2-amidinopropane) (AAPH) Radicals, Hydrogen Peroxide and tert-Buthylhydroperoxide by Capillary Zone Electrophoresis

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Twelve major anthocyanins identified in bilberry extracts were studied in vitro using capillary zone electrophoresis (CZE) for their reactions towards 2, 2'-azobis (2-amidinopropane) (AAPH) radicals, hydrogen peroxides (H_2O_2) and tert-buthylhydroperoxides (t-BuOOH). Reactivity towards AAPH radicals was primarily determined by the aglycon structure, not by the type of sugar moiety. Delphinidins carrying three-hydroxyl groups on the B ring were most reactive followed by cyanidins, with two-hydroxyl groups. Further, methylation of the hydroxyl groups reduced reactivity towards AAPH radicals. However, reactivity of anthocyanins towards H_2O_2 was not significantly affected by aglycon structure or by the type of sugar moiety ; there being no marked difference in reaction rates among the anthocyanins. Reactivity towards t-BuOOH was essentially the same as towards H_2O_2, although the reaction rate was several times smaller. Also, the reaction rate of anthocyanin towards peroxide was relatively high compared to that of (+) -catechin (approximately 30 times larger) measured as a reference antioxidant, whereas the reactivities of anthocyanins and (+) -catechin towards AAPH radicals were similar.
公益社団法人日本薬学会の論文
2004-04-01