Prevention of Oxidative Injury in PC12 Cells by a Traditional Chinese Medicine, Shengmai San, as a Model of an Antioxidant-Based Composite Formula

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The protective effect of Shengmai San (SMS) on oxidative damage in cultured PC 12 cells was studied as a model of an antioxidant-based composite formula usable for the treatment of oxidative stress-related complex disorders. SMS, a traditional Chinese herbal medicine, has previously been shown to prevent cerebral oxidative injury in rats. Neuronal model PC12 cells were incubated with SMS for defined periods, chased with H_2O_2 for 30 min at 37℃, and subjected to an ELISA-based assay for determining the protein carbonyl content, and a Comet assay for DNA single strand breaks (SSBs). The results showed that both protein carbonyl content and DNA SSBs increased in PC12 cells after the H_2O_2 chase in a concentration-dependent manner. Both H_2O_2-dependent carbonyl formation and DNA damage were markedly prevented in the cells pretreated with SMS, and the SMS effects were dependent on both the SMS concentration and the period of pre-incubation with SMS before the H_2O_2 abuse. At the same time, cell viability was enhanced in the SMS-pretreated cells after the H_2O_2 abuse compared to the control cells as determined by an 3-(4,5- (MTT) assay. It is concluded that SMS functions not only as a simple antioxidant but also as a modulator of cellular antioxidant defense.
公益社団法人日本薬学会の論文
2003-07-01