Effect of Chlorinated Ethylenes on the Expression of Rat CYP2C
スポンサーリンク
概要
- 論文の詳細を見る
Chlorinated ethylenes (CEs) are known to be metabolized by CYP2C as well as CYP2E1 and CYP2B. The effects of CEs on the expression of the rat CYP2C gene in the liver and lung were comparatively studied in terms of the enzyme activity, and protein and mRNA contents. Tetrachloroethylene (PCE), trichloroethylene (TCE), and 1,1-dichloroethylene (1,1-DCE) were injected at 0.5 g/kg i.p. into male 7-week-old Wistar rats individually or in combination with phenobarbital (PB). The expression of CYP2C mRNA showed organ-specific properties without being detectable in the lung under the assay conditions employed in the present study. Individual CEs had no effect on the enzymatic activity of CYP2C estimated by 2α-hydroxylation of testosterone (2α-TSH), whereas PB markedly enhanced the enzymatic activity in the liver but not the lung microsomes, although it was highly susceptible to the suppressive effect of 1,1-DCE. A down-regulation of pulmonary enzyme activity was observed with PCE by an unknown mechanism when the animals were treated simultaneously with PB. 1,l-DCE was found to inhibit the expression of hepatic mRNA irrespective of the coexistence of PB, and the same was true for CYP2C protein in the same organ to a lesser extent. In an analogy to our previous findings with other xenobiotic-metabolizing CYP forms such as CYP2B1/2 and CYP2E1, a potent suppressive effect of 1,1-DCE was observed with the maximum around 18 hr after the treatment on both constitutive and PB-induced expression of CYP2C.
- 社団法人日本薬学会の論文
- 2002-12-01
著者
-
Inouye Yoshio
Faculty of Pharmaceutical Sciences, Toho University
-
Mizuno Masaki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Toho University
-
Mizuno Masaki
東邦大学 薬学部公衆衛生学
-
Nakahama Takayuki
School of Pharmaceutical Sciences, Toho University
-
Mizuno Masaki
School of Pharmaceutical Sciences, Toho University
-
Sekiguchi Minori
School of Pharmaceutical Sciences, Toho University
-
Sakamaki Hiroki
School of Pharmaceutical Sciences, Toho University
-
Inouye Yoshio
School of Pharmaceutical Sciences, Toho University
-
Nakahama Takayuki
Fac. Of Pharmaceutical Sciences Toho Univ.
-
Nakahama Takayuki
Faculty Of Pharmaceutical Sciences Toho University
-
Nakahama Takayuki
School Of Pharmaceutical Sciences Toho University
-
Nakahama Takayuki
Faculty Of Pharmuceutical Sciences Toho University
-
Inouye Yoshio
Fac. Of Pharmaceutical Sciences Toho Univ.
-
Inouye Yoshio
School Of Pharmaceutical Sciences Toho University
-
Inouye Yoshio
科学警察研究所
-
Inouye Y
Faculty Of Pharmuceutical Sciences Toho University
-
MOCHIZUKI Megumi
Faculty of Pharmaceutical Sciences, Toho University
-
Sakamaki Hiroki
School Of Pharmaceutical Sciences Toho University
-
Sekiguchi Minori
School Of Pharmaceutical Sciences Toho University
-
Nakahama Takayuki
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Toho University
-
Inouye Y
Faculty Of Pharmaceutical Sciences Toho University
-
Inouye Yoshio
School Of Pharmaceutical Science Toho University
-
Mochizuki Megumi
Department Of Environmental Health Faculty Of Pharmaceutical Sciences Toho University
関連論文
- 5-Aminoimidazole-4-carboxamide-1-β-ribofuranoside (AICAR) prevents nuclear translocation of constitutive androstane receptor by AMP-activated protein kinase (AMPK) independent manner
- A consecutive three alanine residue insertion mutant of human CAR: a novel CAR ligand screening system in HepG2 cells
- A consecutive three alanine residue insertion mutant of human CAR : a novel CAR ligand screening system in HepG2 cells
- Accentuated Antagonism in Vagal Heart Rate Control Mediated through Muscarinic Potassium Channels
- Upright Tilt Resets Dynamic Transfer Function of Baroreflex Neural Arc to Minify the Pressure Disturbance in Total Baroreflex Control
- Baroreflex Increases Correlation and Coherence of Muscle Sympathetic Nerve Activity (SNA) with Renal and Cardiac SNAs
- Transcriptional Determination of Sexually Dimorphic Expression of Nuclear Receptor Constitutively Active Receptor (CAR) in Wistar Rats
- Effect of Chlorinated Ethylenes on the Expression of Rat CYP2C
- Comparative Study on Correlation between Chemical Structure and Effect on Expression of Cytochrome P450 mRNAs in Rat among Chlorinated Ethylenes, Tetrachloroethylene, Trichloroethylene, 1, 1-Dichloroethylene
- Comparative Study on the Mode of Action of Chlorinated Ethylenes on the Expression of Rat CYP Forms
- Expression Analysis of Estrogen-responsive Genes Vitellogenin 1 and 2 in Liver of Male Medaka (Oryzias latipes) Exposed to Selective Ligands of Estrogen Receptor Subtypes
- 5-Aminoimidazole-4-carboxamide-1-β-ribofuranoside (AICAR) prevents nuclear translocation of constitutive androstane receptor by AMP-activated protein kinase (AMPK) independent manner
- Biological Evaluation of Pollution in the Sagami River Using the 7-Ethoxycoumarin O-Deethylase Activity Induced by River Sediment Extracts in HepG2 Cells
- Biological Evaluation of the Pollution of the Tsurumi River with 7-Ethoxycoumarin O-Deethylase Activity Induced by River Sediment Extracts in HepG2 Cells
- Feasibility of Bioavailability Testing by Simultaneous Determination of Serum Concentrations of Tegafur and 5-fluorouracil after TS-1 Oral or Tube Administration for Chemotherapy in Oral Cancer Patients
- Expression of Constitutive Androstane Receptor Splice Variants in Rat Liver and Lung and Their Functional Properties(Molecular and Cell Biology)
- The Inhibitory Effect of Aryl Hydrocarbon Receptor Represser (AhRR) on the Growth of Human Breast Cancer MCF-7 Cells(Molecular and Cell Biology)
- Primary Structure and Organ-Specific Expression of the Rat Aryl Hydrocarbon Receptor Represser Gene(Molecular and Cell Biology)
- Possible Involvement of Chlorinated Ethylenes in Arylhydrocarbon Receptor-Related Induction of Cytochrome P4501A1(CYP1A1) in Human Hepatoma HepG2 Cells
- Role of the Defective Splicing of mRNA in the Lack of Pulmonary Expression of Constitutively Active Receptor in Rat
- Biological Evaluation of the Pollution of Rivers Flowing into Tokyo Bay with the 7-Ethoxycoumarin O-deethylase (ECOD) Activity Induced by River Sediment Extracts in HepG2 Cells
- Effects of Chlorinated Ethylenes on Expression of Rat CYP Forms : Comparative Study on Correlation between Biological Activities and Chemical Structures
- Effects of Tetrachloroethylene and 1,1,1-Trichloroethane on the Expression of P450 Isoforms in Rat Lung and Liver
- Comparative Study on the Effect of Trichloroethylene on the Expression of P450 Isoforms in Rat Lung and Liver(PROCEEDINGS OF 24TH SYMPOSIUM ON TOXICOLOGY AND ENVIRONMENTAL HEALTH)
- Surgical Treatment of Lumbar Canal Stenosis in the Elderly
- (17α,20E)-17,20-[(1-Methoxyethylidene)bis(oxy)]-3-oxo-19-norpregna-4,20-diene-21-carboxylic Acid Methyl Ester (YK11) Is a Partial Agonist of the Androgen Receptor
- Antitumor Effect of an Adeno-associated Virus Vector Containing the Human Interferon-β Gene on Expertimental Intracranial Human Glioma
- Specificity in the Metabolic Activation of Chlorinated Ethylenes by Cytochromes P450 in Primary Rat Hepatocytes
- Divergent Modes of Induction of Rat Hepatic and Pulmonary CYP3A1 by Dexamethasone and Pregnenolone 16α-Carbonitrile
- Excretory Profile of 4-Bromo-2,5-dimethoxy-phenethylamine (2C-B) in Rat
- Mode of Action of Chlorinated Ethylenes on the Expression of Rat Cytochrome P450 forms and Specificity in the Metabolic Activation of CEs by CYPs
- Comparative Study on in vitro Inhibitory Effects of Heavy Metals on Rabbit Drug-Metabolizing Enzymes
- Behavior of Extractable Organic Halogens in a Municipal Solid Waste Incinerator and the Relationship with That of Polychlorinated Dibenzo-p-dioxins and Polychlorinated Dibenzofurans
- EFFECTS OF HEAVY METALS ON THE ACTIVITY OF DRUG-METABOLIZING ENZYMES OF RABBIT LUNGS
- Shock Waves in a van der Waals Gas (Part 3) Two-Dimensional Shock Waves
- Shock Waves in a van der Waals Gas (Part2) One-Dimensional Shock Waves