Mechanisms Underlying Regulation of Local Immune Responses in the Uterus during Early Gestation of Eutherian Mammals II. Electron Microscopic Studies in the Interactions between Blastocysts and Macrophages Cultured Together in vitro(Immunology)

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Mouse blastocysts were cultured together with macrophages of allogeneic origin in vitro. Observations were made approximately 1.5, 6.0, 26.0, 48.0, 72.0 and 96.0 hr after the initiation of the experiment. Many macrophages were present on the zonae pellucidae of the cultured blastocysts particularly during the period prior to, and in the process ofzona-shedding. Hatching of the blastocysts from the zonae as well as spreading of trophoblast cells took place without any recognizable signs of unusual development in the presence of the phagocytes. The trophoblast cells exhibited no morphologically explicit cytotoxicity toward the macrophages, majority of which appeared to be simply pushed aside physically by force exerted by the trophoblast cells undergoing spreading. Occasionally phagocytized cells which were probably macrophages, were found within the cytoplasm of the trophoblast cells. Macrophages were often observed to have insinuated into the inner cell mass (ICM) and attached to the ICM cells. It was not possible in the present series of experiments, to ascertain whether the macrophages were cytotoxic to the ICM cells or not.
社団法人日本動物学会の論文
1985-10-15