Orientation of Smooth Muscle-Derived A10 Cells in Culture by Cyclic Stretching : Relationship between Stress Fiber Rearrangement and Cell Reorientation(Cell Biology)
スポンサーリンク
概要
- 論文の詳細を見る
Mechanical stress causes various responses in cells both in vivo and in vitro. Realignment of cells and stress fibers is one of the remarkable phenomena that are induced by the stress. However, the mechanism by which their realignment is controlled is largely unknown. In this study, effects of mechanical stretch on the morphology of cultured cells were examined using a cyclic and reciprocal cell stretching apparatus. A10 cells, a cell line derived from rat aortic smooth muscle, were used as a model, since they are spindle-shaped and have remarkable stress fibers aligned along the longitudinal cell axis. Therefore, the orientation of the cell and stress fibers could be easily identified. When the cells were cultured on elastic silicone membranes and subjected to cyclic and reciprocal stretch with an amplitude of 20% at a frequency of 60 cycles per minute, actin stress fibers were aligned obliquely to the direction of stretching with angles of 50 to 70 degrees within about 15 min after the onset of stretching. Then, after 1-3 hr of cyclic stretching, the long axes of a majority of the cells were also reoriented to similar directions to the stress fibers. The stretchinduced cell reorientation was blocked by 1 μM cytochalasin B, but not by colcemid. These results indicate that the orientation of cells and actin filaments are closely related and actin filaments play a critical role in the early step of the cell reorientation.
- 社団法人日本動物学会の論文
- 2000-07-01
著者
-
Hayakawa Kimihide
Department Of Biology Faculty Of Science Chiba University:graduate School Of Science And Technology
-
Hayakawa Kimihide
Department Of Biology Faculty Of Science Chiba University
-
Hosokawa Atsushi
Department Of Dermatology Faculty Of Medicine University Of The Ryukus
-
Hosokawa Atsushi
Department Of Biology Faculty Of Science Chiba University
-
Obinata Takashi
Department Of Biology Faculty Of Science Chiba University
-
Yabusaki Katsumi
Department of Biology, Faculty of Science, Chiba University
-
Yabusaki K
Chiba Univ. Chiba Jpn
-
Yabusaki Katsumi
Department Of Biology Faculty Of Science Chiba University:(present Address)biosignal Engineering Div
-
Obinata Takashi
Department Of Biology Faculty Of Science Chiba University:graduate School Of Science And Technology
-
Obinata Takashi
Department Of Biological Sciences Graduate School Of Science University Of Tokyo : Department Of Bio
関連論文
- Effects of BTS (N-benzyl-p-toluene sulphonamide), an Inhibitor for Myosin-Actin Interaction, on Myofibrillogenesis in Skeletal Muscle Cells in Culture
- Case of chromoblastomycosis appearing in an Okinawa patient with a medical history of Hansen's disease
- CLONING OF A NOVEL ISOFORM OF PARVALBUMIN cDNA FROM THE SKELETAL MUSCLE OF THE BULLFROG, RANA CA TESBEIANA(Endocrinology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Low Molecular-weight G-actin Binding Proteins Involved in the Regulation of Actin Assembly during Myofibrillogenesis
- CLINICAL FINDINGS OF CUTANEOUS LEISHMANIASIS AND THEIR DIFFERENTIAL DIAGNOSIS IN ECUADOR
- Pre-exposure with low-dose UVA suppresses lesion development and enhances Th1 response in BALB/c mice infected with Leishmania (Leishmania) amazonensis
- OVER-EXPRESSION OF V-1/MYOTROPHIN INDUCES CARDIAC HYPERTROPHY IN CULTURED CHICKEN CARDIAC MYOCYTES(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- A Preliminary Study Aimed at the Detection of Leishmania Parasites in Subjects with Cutaneous Leishmaniasis Using Polymerase Chain Reaction
- ORAL TREATMENT OF NEW WORLD CUTANEOUS LEISHMANIASIS WITH ANTI-MALARIAL DRUGS IN ECUADOR : A PRELIMINARY CLINICAL TRIAL
- Phaeohyphomycosis caused by Exophiala jeanselmei in a patient with polymyalgia rheumatica
- Temporal and Spatial Expression of Distinct Troponin T Genes in Embryonic/Larval Tail Striated Muscle and Adult Body Wall Smooth Muscle of Ascidian
- Stimulus-dependent Disorganization of Actin Filaments Induced by Overexpressionof Cofilin in C2 Myoblasts
- Assembly of Cardiac C-protein during Myofibrillogenesis in Myogenic Cells in Culture
- COMPARATIVE STUDIES ON THE ROLE OF COFILIN/ADF PROTEINS IN MYOFIBRILLOGENESIS DURING AVIAN MUSCLE DEVELOPMEN(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- A Clinical and Bacteriological Examination of Mycobacterium leprae in the Epidermis and Cutaneous Appendages of Patients with Multibacillary Leprosy
- DISORGANIZATION OF MYOFIBRILS IN COFILIN-DEFICIENT SKELETAL MUSCLE CELLS(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Differential Assembly of Cytoskeletal and Sarcomeric Actins in Developing Skeletal Muscle Cells In Vitro
- Comparative Studies of the Detection Rates of Leishmania Parasites from Formalin, Ethanol-fixed, Frozen Human Skin Specimens by Polymerase Chain Reaction and Southern Blotting
- A case of tinea nigra palmaris in Okinawa, Japan
- N-TERMINAL DOMAINS OF CHICKEN CARDIAC MyBP-C (C-PROTEIN) IS INVOLVED IN BINDING TO ACTIN FILAMENTS(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- FUNCTIONAL CHARACTERISTICS OF TROPONIN IN AMPHIOXUS STRIATED MUSCLE(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- A NOVEL VARIANT OF CARDIAC MYOSIN-BINDING PROTEIN-C THAT IS UNABLE TO ASSEMBLE INTO SARCOMERES IS EXPRESSED IN THE AGED MOUSE ATRIUM(Physiology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Dephosphorylation of Cofilin in Parotid Acinar Cells
- Immunochemical Studies of an Actin-binding Protein in Ascidian Body Wall Smooth Muscle
- Antiserum against the Synthetic Polypeptide Fragment of Dystrophin Cross-reacts with Myofibrillar C-protein
- PARVALBUMIN EXPRESSION IN THE HINDLIMB MUSCLE OF THE POSTMETAMORPHIC BULLFROG, RANA CATESBEIANA
- Developmental Relationship of Myosin Binding Proteins (Myomesin, Connectin and C - Protein) to Myosin in Chicken Somites as Studied by Immunofluorescence Microscopy
- Orientation of Smooth Muscle-Derived A10 Cells in Culture by Cyclic Stretching : Relationship between Stress Fiber Rearrangement and Cell Reorientation(Cell Biology)
- SUPPRESSION OF COFILIN EXPRESSION BY ANTI-SENSE MORPHOLINO OLIGO NUCLEOTIDES AND MYOFIBRILLOGENESIS IN CULTURED SKELETAL MUSCLE CELLS(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japa
- Immunocytochemical Localization of Troponin I and C in the Muscles of Caenorhabditis elegans
- Effects of Exogenous β-Actinin(CapZ)on Actin Filamentous Structures in Cultured Muscle Cells
- Primary Structure of Mouse Actine-Related Protein 1 (Arp1) and Its Tissue Expression
- Increased Expression of Cofilin in Denervated Chicken Skeletal Muscle
- Transitions of Myofibrillar Protein Isoforms and Their Assembly in Developing Muscle Cells(THE ZOOLOGICAL SOCIETY PRIZE)(Proceedings of the Fifty-Fifth Annual Meeting of the Zoological Society of Japan)
- Molecular process of myofibrillogenesis : roles of cofilin and C-protein in the assembly and maintenance of myofibrils(Symposium on Current Research for Skeletal Muscle)
- Uptake of Albumin is Coupled with Stretch-Induced Hypertrophy of Skeletal Muscle Cells in Culture(Cell Biology)
- Influence of Minor Trabecular Elements on Fast and Slow Wave Propagations through Cancellous Bone
- Effect of Cortical Bone Layer on Fast and Slow Waves in Cancellous Bone: Investigations Using Stratified Models
- Effect of Trabecular Irregularity on Fast and Slow Wave Propagations through Cancellous Bone
- Ultrasonic Pulse Waves Propagating through Cancellous Bone Phantoms with Aligned Pore Spaces
- Effect of Minor Trabecular Elements on Fast and Slow Wave Propagations through a Stratified Cancellous Bone Phantom at Oblique Incidence