Uptake of Albumin is Coupled with Stretch-Induced Hypertrophy of Skeletal Muscle Cells in Culture(Cell Biology)
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概要
- 論文の詳細を見る
Hypertrophy is induced in skeletal muscle when mechanical overload, for example repetitive stretching, is presented. This is a well-known phenomenon and the molecular mechanism involved has been investigated from various aspects. In this study, with a system that enables periodic stretching of cultured skeletal muscle cells, myotubes, along the long cellular axis uni-directionally at a constant frequency, we examined the effects of stretching on skeletal muscle using mouse C2 myotubes in culture as a model. Significant hypertrophy was observed in the myotubes after several days of periodic stretching and this was accompanied by the accumulation of a protein of about 67kDa. This protein was identified with albumin, which was present in the culture medium, based on its antigenicity, size and pi. When bovine serum albumin tagged with biotin was added to the culture medium, it became detectable in the cytoplasm of the stretched myotubes. mRNA encoding albumin was not detectable in the myotubes by northern blotting irrespective of their stretching or non-stretching, indicating that transcription of the albumin gene was not induced in the stretched muscle cells. From these results, we conclude that the accumulation of albumin in stretched myotubes was due to uptake of the protein from the culture medium not to de novo synthesis of the protein in myotubes. We suggest that albumin uptake may be involved in skeletal muscular hypertrophy.
- 社団法人日本動物学会の論文
- 2003-05-25
著者
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Obinata Takashi
Teikyo‐heisei Univ. Tokyo Jpn
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Adachi R
Department Of Biology Faculty Of Science Chiba University:(present Address)department Of Biology Fac
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Yabusaki Katsumi
Department Of Biology Faculty Of Science Chiba University:(present Address)biosignal Engineering Div
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Obinata Takashi
Department Of Biological Sciences Graduate School Of Science University Of Tokyo : Department Of Bio
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Adachi Ryota
Department of Biology, Faculty of Science, Chiba University
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Adachi Ryota
Department Of Applied Chemistry Faculty Of Science And Technology Keio University
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