媒液中のカルシウムの存在によるイモリ遊離割球の分裂能の保持

概要

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When egg fragments taken from the animal region of the middle blastulae of the newt, Cynopus pyrrhogaster, are exposed to solutions such as Ca-free Holtfreter's solution (CFHS), CFHS plus Na-citrate, or Sorensen phosphate mixture M/15 pH 7.8, dissociation of constituent blastomeres takes place in about one hour at ca. 25℃. In the dispersed condition division of he blastomere is generally inhibited, but in some cases the blastomeres are seen to divide only once or twice. Contrary to this, in media containing calcium at appropriate concentrations the blastomeres remain viable for a considerable length of time and continue to divide. When placed in standard Holtfreter's solution in which calcium concentration is reduced to 1/40 of the original value, each isolated blastomere, after repeated cell divisions, attains the size of the cells in a whole embryo at the early gastrula stage.
社団法人日本動物学会の論文
1974-03-25