イトミミズの卵割溝にみられる分泌物について

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概要

• 論文の詳細を見る

• In cleaving eggs of aquatic oligochaetes, Tubifex hattai and Limnodrilus willeyi, dark blue spots stained with Muller-Hale's colloidal iron method for acid mucopolysaccharide were observed near the furrow region. The preservation of the mucosubstance could be influenced considerably by different fixing reagents. Alcohol-containing and acidic fixatives had a deleterious effect upon the preservation of the substance. Non-acidic formalin and iodate- or dichromate-containing formalin solution gave satisfactory results. A pH above 6.0 generally seemed to favour the fixation of the substance. An accumulation of colloidal iron-positive granules was observed periodically in parallel with the mitotic cycle of the first and the second cleavages of the egg. Prior to the formation of cleavage furrow the cytoplasm showed a weak blue reaction as a whole, while blue spot on the surface of the egg appeared simultaneously with furrowing of the first cleavage. Further, the formation of the spots began first at the furrow, and spread to the pole region of the egg. After the emergence of the spots the blue color of the cytoplasm disappeared. Then, the number of the spots started decreasing, and when the two blastomeres flattened adhering to each other, two or three of large blue spots were observed near the furrow. The cytoplasm at this time showed a weak blue reaction. In the second cleavage, the same processes were repeated although the blue-colored blastomeres and spotted blastomeres with white-colored cytoplasm were variously in mosaic. In the sectioned specimens of cleaving eggs, colloidal iron-positive granules measuring about 0.5 μ in diameter were accumulating toward egg surfaces, and sometimes densely stained vacuoles were observed inside of the cell membrane. From the present observation it is assumed that the accumulation of the granules in the cytoplasm is followed by the formation of vacuoles at the site of emergence of granules and that the mucosubstance of the vacuoles is secreted into the perivitelline space.

• 社団法人日本動物学会の論文
• 1968-02-15

著者

• 松本 政美

  山形大学理学部生物学科

関連論文

• イトミミズ卵の成熟分裂および初期卵割における経過時間測定
• イトミミズTubifex hattai の二形卵から孵化した個体の個別飼育
• 飼育条件下における水棲貧毛類Tubifex hattaiの卵二形について
• 室内飼育による水棲貧毛類Tubifex hattaiの生長と産卵数に対する餌量の影響
• 水棲貧毛類Tubifex hattaiの生活史
• イトミミズの胚分泌物に対するFe(II)─Fe(III)の親和性(生化学)
• イトミミズの胚分泌物に対する鉄の親和性(発生)
• イトミミズ胚の卵割溝における分泌物の組織化学的反応
• イトミミズの卵割溝にみられる分泌物について
• イトミミズの胚における顕微灰像
• 卵形の変化を中心にしたイトミミズ卵の成熟分裂および初期卵割の観察
• 水棲貧毛類Tubifex hattaiの産卵量の季節的変動について

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