DES処理によって雌性化された鶏胚生殖巣の性変更能力

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The feminized left gonad of the 11-day male chick embryo which was induced by dipping eggs on the 4th day of incubation into the diethylstilbestrol (DES) solution (50 mg DES/100 ml of isopropyl alcohol) was cultured for five days in association with the sex indifferent left gonad of the intact 8-day male embryo. The culture method contrived by Et. Wolff (1961) was employed, in which the vitelline membrane of the unfertilized egg was utilized as a substratum and as an intermediary membrane between explants (Fig. 1). As the results, the gonad derived from the intact male embryo was modified into an intersexualized structure which consisted of abnormally hypertrophied epithelium and developing testicular cords; the hypertrophied epithelium was comparable to the cortex of the primordial ovary (Figs. 2-6). In the sections of the intersexualized gonad which were stained with acridine orange and examined by ultraviolet ray activation, an evident increase of RNA inclusions in the cytoplasm was detected (Figs. 7, 8). Considering collectively the results obtained and those of the foregoing studies, it was concluded that the inductive agent inducing the epithelial hyperplasia could be attributable to the lacunarized medulla of the DES-treated gonad, but the inductive substance responsible for the hyperplasia was not necessarily ascribed to the estrogen produced de novo in the lacunalized medulla; the chemical property of the inductive substance still remains to be clarified by further studies.
1967-08-15

DES処理によって雌性化された鶏胚生殖巣の性変更能力

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