A Family of Highly Repetitive DNAs from "Ginbuna" (Carassius auratus langsdorfi) Genome Common to Carassius auratus Populations

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A family of highly repetitive DNAs (Hi-a) in the genome from a population of the crucian carp, tentatively identified as the ginbuna (Carassius auratus langsdorfi), was isolated from the HindIII digests and characterized. The Hi-a monomer (268 or 269 bp) was AT-rich (64.9%) with internal repetitive oligomers. The nucleotide similarity among monomers within the same individual was 88-98%, whose sequence alterations occurred mostly at restricted sites. Hybridization analyses revealed that the Hi-a family was organized into tandem array (s) representing satellite DNAs, and that its variants that share certain restriction sites of the repeat unit were dispersed into the tandem array (s) of Hi-a DNAs at varying periodicities. The lack of sequences homologous to the ginbuna Hi-a in the genomes from cyprinid fishes other than gengorobuna and goldfish suggests that Hi-a DNAs are specifically present in the species C. auratus. The genome of goldfish differed somewhat in the quaantity or the genomic organization of Hi-a DNAs from that of the ginbuna.
社団法人日本動物学会の論文