Characterization of repetitive DNA sequences carrying 5S rDNA of the triploid ginbuna (Japanese silver crucian carp, Carassius auratus langsdorfi

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概要

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• Repetitive DNA sequences (Hi-b; 209 bp in length) were isolated from the HindIII digests of the genomic DNA of the triploid ginbuna, Carassius auratus langsdorfi. Sequence analyses revealed that the Hi-b repetitive units were comprised of the complete coding regions of 5S rDNA (120 bp in size) and their 5 flanking regions. The sequences of the Hi-b units from the same individual were highly homogeneous. Southern blot hybridization to the Hi-b probe displayed intricate patterns that represented the presence of other repetitive units containing the Hi-b related sequences. A major family of repetitive sequences related to the Hi-b was then obtained by the polymerase chain reaction using asymmetry primers for the 5S coding regions. These 331-bp sequences (AZ5Ss) contained 5S pseudogenes as well as the almost entire Hi-b sequences, and seemed to be the true 5S rDNAs. The tandem arrangements of the AZ5S sequences explained most of the complex results of Southern blots. Another class of intriguing repeat units (Hi-b-β and Hi-b-γ) were also isolated. Fluorescence in situ hybridization data revealed two major signals on a pair of homologous chromosomes and several minor signals on other chromosomes in the triploid ginbuna, indicating the existence of the 5S related sequences as several separate clusters. The major spots were shared with the tetraploid ginbuna and goldfish, but not with the diploid ginbuna. When the genomic organization of the Hi-b related sequences in other cyprinid fishes was examined, the hybridization patterns of the ginbuna were very simillar to those of the goldfish, but were clearly different from those of the gengorobuna. The carp genome showed less complex patterns. Thus, the present 5S rDNA-related sequences could be candidates for phylogenetic molecular markers for the crucian carp.

• 日本遺伝学会の論文

著者

• Fujitani Hideo

  Laboratory Of Molecular Biology Azabu University School Of Veterinary Medicine

• Murakami M

  Laboratory Of Molecular Biology Azabu University School Of Veterinary Medicine

• Murakami Masaru

  Laboratory of Molecular Biology, Azabu University School of Veterinary Medicine

• Murakami Masaru

  Laboratory Of Molecular Biology Azabu University School Of Veterinary Medicine

関連論文

• A Family of Highly Repetitive DNAs from "Ginbuna" (Carassius auratus langsdorfi) Genome Common to Carassius auratus Populations
• Characterization of repetitive DNA sequences carrying 5S rDNA of the triploid ginbuna (Japanese silver crucian carp, Carassius auratus langsdorfi
• HYPOLIPIDEMIC EFFECT AND ENHANCEMENT OF PEROXISOMAL β-OXIDATION IN THE LIVER OF RATS BY SODIUM-(E)-3-(4-(3-PYRIDYLMETHYL) PHENYL)-2-METHYL PROPENOATE (OKY-1581), A POTENT INHIBITOR OF TxA_2 SYNTHETASE
• The maternal origins of the triploid ginbuna (Carassius auratus langsdorfi): phylogenetic relationships within the C. auratus taxa by partial mitochondrial D-loop sequencing
• Polyploid-specific repetitive DNA sequences from triploid ginbuna(Japanese silver crucian carp, Carassius auratus langsdorfi)
• The Complete Sequence of Mitochondorial Genome from a Gynogenetic Triploid "Ginbuna"(Carassius auratus langsdorfi)
• Determination of Full-Length cDNA Nucleotide Sequence of Equine Carbonic Anhydrase VI and Its Expression in Various Tissues
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