Cryofiltrationにおける最近の技術的動向

概要

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Cryofiltration is a double filtration plasmapheresis (DFPP) and, besides the DFPP system, requires the cooling system and the washing-out procedure of the second filter for reuse. We developed the cooling system. At first, we soaked the cooling coil and second filter in ice and water, which was simplest, but it was difficult to control the temperature. The second version of the cooling system was an electric refrigerator, which required more than one-and-a-half hours to get ready. The coolers capacity was 55L. The third version was soaking the coil in propylene glycol, which was the cooling medium. It required one hour to prepare. To make the cooling system smaller, we adopted the Pertier element for cooling. A thin cooling bag was sandwiched between the two cooling plates, which consisted of 13 pieces of Pertier element. This system could attain a constant temperature within 5 min. The washing-out procedure of the second filter was so complicated that we devoloped an automatic washing-out system with computer control. The automatic cryofiltration system, which consists of the cooling plate system and the automatic washing-out system, is useful not only for cryofiltration, but also for DFPP and adsorption apheresis.
日本アフェレシス学会の論文
2002-02-28