IMMUNOHISTOCHEMICAL LOCALIZATION OF METALLOTHIONEIN IN THE LIVER AND KIDNEY OF CADMIUM- OR ZINC-TREATED RATS

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概要

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• The localization of metallothionein (MT), a small molecular weight heavy-metal binding protein, in the liver and kidney of rats treated with either cadmium (Cd) chloride or zinc (Zn) sulfate was investigated by the indirect immunofluorescent technique. Male Wistar rats were subcutaneously injected with CdCl_2 at a single dose of 3.O mg Cd/kg body weight or 50 mg Zn/kg body weight, followed by sacrifice at 3, 6, 12 and 24 hr. For comparison, tissues were also obtained from rats treated with 1.5 mg Cd/kg body weight, 4 times a week for 6 weeks. In control rats from both short- and long-term studies the MT antigenicity was detected in the cytoplasm of hepatocytes and proximal tubular cells. A major difference in the localization of MT between the two studies was found only in the liver. The immunoreactive stain was limited to the hepatocytes around both central and interlobular veins in the long-term study compared to rather uniform localization throughout the liver in the short-term study. The intensity of immunostain of MT in both organs upon Cd or Zn treatment tended to incrcase with tisrue concentrations of the protein, as determined by a radioinmunoassay. Cd and Zn treatment resulted in the localization of MT in a similar manner. In particular, the number of proximal tubules with immunofuorescence increased upon an injection of Cd or Zn, and once a renal Cd level considerably incrcased by the repeated injections of Cd, intense stain of MT was observed not only in the proximal tubule but also the distal tubule and collecting duct. No specific immunostain was observed in the glomerulus, vascular endothelial cells and connective tissucs. Although not only the eytoplasm but also the nucleus of the hepatocutes and proximal tubular cells often showed immunofluorescencc, no significant change in the induction of MT betwecn these subcellular compartments with time was found.

• 日本組織細胞化学会の論文

著者

• Tohyama Chiharu

  Environmental Health Sciences Division, National Institute for Environmental Studies

• NISHIMURA Noriko

  Department of Clinical Sciences and Laboratory Medicine

• Tohyama Chiharu

  Division Of Environmental Health Sciences Center For Disease Biology And Integrative Medicine Gradua

• Tohyama Chiharu

  Environmental Health Sciences Division National Institute For Environmental Studies:department Of Hy

• Tohyama Chiharu

  Environmental Health Sciences Division National Institute For Environmental Studies:center For Disea

• Tohyama Chiharu

  Environmental Health Science Division National Institute For Environmental Studies

• Nishimura Hisao

  Department of Hygiene, Aichi Medical University

• Tohyama C

  Environmental Health Sciences Division National Institute For Environmental Studies:center For Disea

• Nishimura Hisao

  Environmental Health Sciences Division National Institute For Environmental Studies:department Of Hy

• Nishimura Hisao

  Department Of Hygiene Aichi Medical University

• Nishimura Noriko

  Res. Center For Environmental Risk National Inst. For Environmental Studies

• Tohyama Chiharu

  Environ Health Sci Div. Nat'l Inst Environ. Studies

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