EVALUATION OF DESTAINING METHODS IN DNA CYTOFLUOROMETRY OF THE WRIGHT-GIEMSA STAINED NORMAL AND LEUKEMIC CELLS
スポンサーリンク
概要
- 論文の詳細を見る
In order to determine DNA content of leukemic cells by means of cytofluorometry, morphological identification of cells on the Wright-Giemsa (W-G) stained smear is required prior to Feulgen nuclear reaction. The complete removal of W-G staining is an essential problem to perform accurate DNA cytofluorometry. This paper reports the most reliable destaining method which preserves the proportionality between fluorescence intensity and DNA content based on a comparative study of several conventional destaining methods. The destaining method with 5% TCA followed by 100% methanol is considered to be the most practical and reliable method to remove W-G staining on the accurate DNA cytofluorometry of the cells identified by W-G stain.
- 日本組織細胞化学会の論文
著者
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Nakagawa Masao
2nd Dept. Anat. Kyoto Pref. Univ. of Med.
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Shimazaki C
Department Of Molecular Hematology And Oncology Graduate School Of Medical Science Kyoto Prefectural
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Shimazaki Chihiro
Division Of Hematology And Oncology Department Of Medicine Graduate School Of Medical Science Kyoto
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Shimazaki Chihiro
京都府立医科大学 循環器内科学･腎臓内科学
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Shimazaki Chihiro
Second Department Of Internal Medicine Kyoto Prefectural University Of Medicine
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Shimazaki Chihiro
京都府立医科大学
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Shimazaki Chihiro
Department Of Molecular Hematology And Oncology Graduate School Of Medical Science Kyoto Prefectural
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NAKAGAWA Masao
the Second Department of Medicine, Kyoto Prefectural University of Medicine
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HARUYAMA Harue
Department of Medicine, Shakai-hoken Kyoto Hospital
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SHIMAZAKI Chihiro
2nd Department of Internal Medicine, Kyoto Prefectural University of Medicine
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HARUYAMA Harue
2nd Department of Internal Medicine, Kyoto Prefectural University of Medicine
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ISEMURA Takuji
2nd Department of Internal Medicine, Kyoto Prefectural University of Medicine
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IJICHI Hamao
2nd Department of Internal Medicine, Kyoto Prefectural University of Medicine
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NAKANISHI SHINOBU
2nd Department of Internal Medicine, Kyoto Prefectural University of Medicine
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Haruyama Harue
Department Of Medicine Shakai-hoken Kyoto Hospital
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Isemura Takuji
2nd Department Of Internal Medicine Kyoto Prefectural University Of Medicine
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Nakanishi Shinobu
2nd Department Of Internal Medicine Kyoto Prefectural University Of Medicine
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Ijichi Hamao
2nd Department Of Internal Medicine Kyoto Prefectural University Of Medicine
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SHIMAZAKI Chihiro
Kyoto Prefecture University of Medicine
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Nakagawa Masao
The Second Department Of Medicine Kyoto Prefectural University Of Medicine
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Shimazaki Chihiro
Division Of Hematology And Oncology Department Of Medicine Graduate School Of Medical Science Kyoto Prefectural University Of Medicine
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Nakagawa Masao
2nd Department of Medicine, Kyoto Prefectural University of Medicine
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