FERRITIN IN MACROPHAGES OF THE MOUSE LYMPH NODE. 200 KV ELECTRON MICROSCOPY AND X-RAY MICROANALYSIS USING FRESH PRINT PREPARATION
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For preservation of the cellular relationships in the specimens, freshly cut edges of mouse lymph nodes were lightly contacted onto collodion membrane covering copper grids. Air-dried spreads and those fresh frozen dried were examined by high resolution electron microscopy. Macrophages surrounded by mononuclear cells had irregular large nucleus and lucent cytoplasm containing a number of homogeneous dense bodies, mitochondria with fine dense granules and peripheral vacuoles. At higher magnification, several homogenous dense bodies were disclosed to be full of ferritin particles. These were also dispersed among ribosomes. Energy dispersive X-ray microanalysis of the homogeneous dense bodies revealed phosphorus, sulphur, chlorine and potassium. That of ferritin containing bodies indicated iron as well as these elements.
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