ELEMENTAL ANALYSIS OF HUMAN PLATELETS ON FRESH AIR-DRIED BLOOD SMEARS BY WAVE DISPERSIVE X-RAY MICROANALYSIS USING STEM EQUIPMENT

概要

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Wave dispersive X-ray microanalysis was performed on platelets and red blood cells of a healthy male and a von Willebrand patient, and mast cells and lymphocytes of the mouse peritoneal fluid. A STEM equipment of target method was modified by replacing grid holders with carbon rod tubes and the reflecting stage with a carbon disc for microanalysis, attempting to decrease the background noises. In secondary electron images at acceleration voltage of 15-45 kV and absorption current of 0.03-0.1 nA, the above cells were recognized and also identified in back scattered electron images at an absorption current of 0.1-0.5 μA by their profiles and relationship with other cells. High peaks of characteristic X-ray counts of Na-, P-, S-, Cl and K-Kα were disclosed in the analysis of cells. Ca- and Mg-Kα were distinctly disclosed in that of the healthy male and Ca- or Mg-Kα was not in that of patient platelets. Analysis of healthy male platelets, mouse mast cells and lymphocytes by means of the pulse height analyzers detected Zn-Kα and Zn-Lα. Electron microscopy of healthy male platelets disclosed the dense bodies but platelets of the patient failed to reveal them.
日本組織細胞化学会の論文