IMMUNOHISTOCHEMICAL LOCALIZATION OF FSH AND LH IN THE HUMAN PITUITARY GLANDS
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概要
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The localization of follicle stimulating hormone (FSH) and luteinizing hormone (LH) in human pituitary gland was studied using the double staining technique of the indirect immunoenzyme method. Human pituitary specimens obtained from 7 adult patients were fixed with 10% buffered formalin solution. Anti-swine FSH (sFSH) and anti-human chorionic gonadotropin-β subunit (hCGβ) antiserum were used for the present investigation. 3,3'-diaminobenzidine (DAB), 4-Cl-1-naphthol and 4-Cl-1-naphthol-pyronine were employed for the substrates of horseradish peroxidase (HRP). FSH containing cells appeared as oval and polygonal type cells, while LH containing cells were almost all oval type, smaller, and fewer in number than FSH containing cells. After decolorization with ethanol and staining with resorcin, fuchsin and azan, FSH and LH containing cells appeared to be Romeis γ-cell and δ-cell, respectively.
- 日本組織細胞化学会の論文
著者
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OKADA KIYOKI
Department of Urology, University of Tsukuba
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Okada Kiyoki
Department Of Urology Nihon University School Of Medicine
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Kawaoi Akira
First Department Of Pathology Nihon University School Of Medicine
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KITAJIMA KIYOAKI
Department of Urology, Nihon University School of Medicine
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Kitajima Kiyoaki
Department Of Urology Nihon University School Of Medicine
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Okada Kiyoki
Department Of Bacteriology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutical Sciences
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