ULTRACYTOCHEMICAL STUDY ON GLUCOSE-6-PHOSPHATASE ACTIVITY IN HUMAN KIDNEY
スポンサーリンク
概要
- 論文の詳細を見る
Glucose-6-phosphatase activity can be demonstrated ultracytochemically in surgical specimen of human kidney fixed by immersion in dilute aldehyde solution. Tissues fixed for 30 min. in 2% glutaraldehyde or 5 min. in 1% paraformaldehyde plus 3% glutaraldehyde solution and incubated for 15 min. in Wachstein-Meisel's medium at 37℃ revealed satisfactory results. The enzyme activity was confined to proximal convoluted tubule cells. Subcellular localizations were smooth and rough endoplasmic reticulum as well as nuclear envelopes. All the plasma membranes including brush borders showed no reaction products. Golgi apparatuses remained unreactive. The enzyme localizations in proximal tubule cells of human kidney were essentially same as those in hepatic parenchymal cells of various animals and in proximal tubule cells of rat kidney in the literature. This investigation showed that G6Pase can be used as a cytochemical marker for the human kidney study.
- 日本組織細胞化学会の論文
著者
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ASO Yoshio
Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University
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OKADA KIYOKI
Department of Urology, University of Tsukuba
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YOKOYAMA MASAO
Toranomon Hospital
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OKADA KIYOKI
Nihon University
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YOKOYAMA Masao
Departments of Urology,Toranomon Hospital
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Aso Yoshio
Department Of Urology Faculty Of Medicine The University Of Tokyo
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Aso Yoshio
Department Of Applied Chemistry Faculty Of Engineering Hiroshima University
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Okada Kiyoki
Department Of Urology Nihon University School Of Medicine
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Obata Koji
Nagoya Dai-ni Red Cross Hospital
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TOKUE Akihiko
Department of Urology, Faculty of Medicine, University of Tokyo
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Tokue Akihiko
Department Of Urology Faculty Of Medicine The University Of Tokyo
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Yokoyama Masao
Department Of Urology Toranomon Hospital
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Aso Yoshio
The Department Of Urology Faculty Of Medicine The University Of Tokyo
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Yokoyama Masao
Department Of Environmental Science Faculty Of Agriculture Tokyo University Of Agriculture And Techn
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Okada Kiyoki
Department Of Bacteriology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutical Sciences
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YOKOYAMA MASAO
Department of Anatomy, Jikei University School of Medicine
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