DOUBLE STAINING OF INDIRECT IMMUNOPEROXIDASE METHOD FOR HCG AND AFP IN THE HUMAN TESTICULAR TUMOR
スポンサーリンク
概要
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The present investigation is aimed at showing the localization of human chorionic gonadotropin (hCG) and alpha-fetoprotein (AFP) in the same section of human testicular carcinoma using successive double staining by the indirect immunoperoxidase method. Specimens from 3 cases of human testicular tumors with elevated serum hCG and AFP values were fixed in 95% ethanol or 10% buffered formalin. Both anti-hCG-β and anti-AFP rabbit sera were absorbed with several materials in order to avoid cross reaction in the double staining. Indirect immunostaining was performed using anti-hCG-β or anti-AFP serum. HRP activity was demonstrated by reacting with either 3, 3'-diaminobenzidine, 4-C1-1 naphthol and 4-C1-1 naphthol-pyronin. HCG- and AFP-localizing cells could be completley differentiated from each other. HCG was localized in the cytoplasm of the syncytiotrophoblastic cells, syncytio-trophoblastic giant cells and embryonal carcinoma cells. AFP was observed at endodermal sinus tumor elements, embryonal carcinoma cells and teratoma elements.
- 日本組織細胞化学会の論文
著者
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OKADA KIYOKI
Department of Urology, University of Tsukuba
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KISHIMOTO Takashi
Department of Molecular Pathology, Chiba University Graduate School of Medicine
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Okada Kiyoki
Department Of Urology Nihon University School Of Medicine
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KITAJIMA KIYOAKI
Department of Urology, Nihon University School of Medicine
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ENDO KATUNORI
Department of Urology, Nihon University School of Medicine
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Endo Katunori
Department Of Urology Nihon University School Of Medicine
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Kitajima Kiyoaki
Department Of Urology Nihon University School Of Medicine
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Kishimoto Takashi
Department Of Urology Nihon University School Of Medicine
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Kishimoto Takashi
Department Of Chemical Science And Engineering Kobe University
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Okada Kiyoki
Department Of Bacteriology Okayama University Graduate School Of Medicine Dentistry And Pharmaceutical Sciences
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