Development of an Enzyme-Linked Receptor Assay (ERA) for hCG

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概要

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• An enzyme-linked receptor assay (ERA) for hCG was developed using horseradish peroxidase. The 1, 500g pellets, interstitial cell fraction, and solubilized homogenate from the rat testis linked to tanned sheep red blood cells (SRBC) were used as the binding fractions for hCG. In these ERA systems, the limit of detection for hCG was 30 IU/<I>l</I> (3-12 mIU/tube), which was almost equal to that of radio receptor assay (RRA). The ERA using SRBC linked solubilized receptor fraction showed the most satisfactory result in accuracy, reproducibility and easiness.

• 社団法人 日本内分泌学会の論文

著者

• Kawaoi Akira

  First Department Of Pathology Nihon University School Of Medicine

• HASHIMOTO MASAKATSU

  First Department of Pathology, Nihon University School of Medicine

関連論文

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• Investigation of New Embedding Mediums for Cytochemistry and Immunoelectronmicroscopy.
• Immunoelectron Microscopic Observation of Serotonin(5HT) Containing Cells in the Rat and Human Intestines
• Immunohistochemical Localization of Steroid Hormones. II. Detection of Progesterone in the Cultured Mouse Adrenocortical Cells (Y-1).
• Development of an Enzyme-Linked Receptor Assay (ERA) for hCG

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